Helical wheel generatorfree

This article is a mix of different solutions, found on the internet or designed specifically for ZMorph VX. Each model was tested and will fit your printer nicely.

The helixvis R package allows users to construct 2-dimensional visualizations of 3-dimensional \(\alpha\)-helical oligopeptides in a programmatic, reproducible manner. Currently, helixvis implements visualization of helical wheels, introduced by @wheeldiag, and wenxiang diagrams, introduced by @wenxiangdiag.

Holding down material for milling and cutting is more difficult and in most cases requires additional equipment. Luckily: ZMorph is both a 3D printer and a CNC unit, so you can always print your workholding solutions!

NOTE: Make sure that the surfaces you want to hold together are clean and dust-free. Otherwise holding force will be much lower.

Helical wheelalpha helix

By default: nonpolar residues (e.g. glycine, tryptophan) will be colored grey; polar residues (e.g. serine, threonine) will be colored yellow; acidic residues with a negative charge at physiologic pH (e.g. aspartic acid, glutamic acid) will be colored red; and basic residues with a positive charge at physiologic pH (e.g. lysine, arginine) will be colored blue. This color scheme can be modified using the col parameter, which accepts a character vector with 4 elements, each of which must be an element of grDevices::colors(). The one-letter code for each residue can also be overlaid on each residue by setting the labels parameter to TRUE.

Two parallel jaws, one movable and one fixed. Sliding mechanism based on screw and lever. Holds down your element to perform operations on it. That’s what a vise is. Its versatility allows mounting almost any part you can think of.

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Another mounting possibility is using step clamps. Step clamps are suited for a variety of material thicknesses, minimizing the number of tools needed.

The following code blocks and figures reproduce the figures from the Helical Wheel section, redrawn as wenxiang diagrams.

NOTE: Default added clamps hold up to 6 mm thick materials. If you have thinner material, there is an available version for up to 2.5 mm thick materials. Print it if needed.

Helical wheel generatoronline free

Using step clamps is very easy. Take the clamp, put it on top of material, add a step underneath it to provide an even surface and tighten down.

Helical wheelprojection online

To fully utilize your ZMorph VX capabilities, you need a mounting solution that won’t reduce your work area. Using this edge vises, you can do just that!

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This is a second guide on CNC milling with ZMorph VX by our expert Roman Broda. Read also how to avoid breaking cutters and metal CNC milling guidelines.

This solution needs a bit of trial and error to find perfect arrangement of your material, but when you do - there’s no other obstacle.

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One of the accessories added to your machine is a set of sheet clamps. They were designed to hold down flat materials up to 6 mm thick. The idea is simple: mount one corner to the CNC table, slide the material into it and block it with second clamp.

Helical wheel generatoronline

The easiest solution for material holding. Simply stick a double sided tape to the bottom of the material and attach it to your worktable. Good quality of the tape will ensure proper holding of your detail during milling and engraving operations. It will also lift your workpiece around 0.5 mm over the table which should be sufficient to not destroy its surface while milling through material.

Helical wheels provide a bird's eye view of an \(\alpha\)-helical peptides and are particularly helpful at highlighting prominent hydrophobic faces to explain a large hydrophobic moment [@eisenberg1982; @dathe1997] and design antimicrobial peptides [@chen2005; @jiang2011].

Now you shall never have problems with material holding again. Choose the best way for your project and start milling. What other solutions do you use?

The following code loads the helixvis package and the sample data that comes with it - these will be used later in this vignette.

How to makehelical wheeldiagram

□ Main Screw Types and Standard Thread Patterns. □ Tool Holder and Insert Selection Guide (SSTE type / SSTI type). □ Threading Method and Insert Angle.

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The vise can be mounted to M4 threaded holes on the sides of your aluminum CNC worktable. Then, using transverse M4 screw and nut, hold down your material. Just remember that there is not much space for adjustments so you will need a material wide enough to use it.

Helical wheel generatorapp

And now you can mount it also on your ZMorph CNC worktable! TheGoofy designed a very neat printable vise that will help you with both cutting and engraving operations. The original design has no possibility to mount it down. Therefore we have uploaded files for mounting it right in the middle of your aluminum CNC table.

Helical wheel generatorcalculator

Wenxiang diagrams are quite similar to helical wheels, with the exception that distance from the first residue is denoted by distance from the center of the spiral. Thus, wenxiang diagrams visually provide the order of amino acids in the sequence. The code used to generate wenxiang diagrams is analogous to that for generation of helical wheels.

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A prototypical \(\alpha\)-helix has approximately 3.6 residues per turn [@helixorig], implying a 100\(^\text{o}\) rotation between consecutive residues. After 18 rotations, the 19th residue returns to the same angle as the 1st residue. Since helical wheels place all residues at an equal radius away from the center, having the same angle of rotation would cause complete overlap of the 1st and 19th residues. As such, helical wheels are limited to plotting 18 residues in a single turn, although some tools do allow for plotting of more than 1 turn [@heliquest]. For consistency, we also limit wenxiang diagrams to 18 residues.

Although additive (3D printing) and subtractive (milling, engraving, cutting) are two opposite manufacturing processes, they have this one thing in common. You have to make sure your material will be held down during the whole process. 3D printing uses all types of tricks, like using paper tape, special surfaces or adhesives. You can learn more about those here.

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